The kit is designed to efficiently remove primers, dNTPs, enzymes, and salts from PCR products in less than 20 minutes. The highly efficient PureLink ® PCR Purification Kit. Store the purified RNA on ice for immediate use. Perform DNase I treatment after purification (refer to the PureLink™ RNA Mini Kit manual) to assure highly pure RNA without genomic DNA contamination. PureLink ® Binding Buffer (B2) PureLink ® Spin Column. • Always mix 1 volume of PCR (50–100 μL) with 4 volumes of Binding : Buffer (B2 or B3). The kit is designed to efficiently isolate genomic DNA from mammalian cells and tissues,mouse tail, E. coli cells, and yeast. The PureLink™ 96 PCR Purification Kit can be used with a vacuum manifold or a centrifuge and is compatible with automated liquid handling workstations (page 6). The PureLink Microbiome DNA Purification Kit minimizes the need to order individual kits for specific sample types because it has been optimized for use with a wide range of biological samples. The kit enables microbial—and, where applicable, host—DNA purification from the following samples: Introduction The PureLink™ Pro 96 PCR Purification Kit is designed for rapid, efficient, and high-throughput purification of PCR products. Isolate and purify high-quality genomic DNA from a variety of DNA types. DNA of up to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl. Find answers to commonly asked genomic DNA purification questions from setting up your purification experiment, performing an extraction, or clean up of a gDNA sample. A full suite of products for all your DNA extraction needs. The kit has several features which are different from other PCR purification systems. Concentrated. PureLink® PCR Purification Kits provide rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR products... Shop Invitrogen™ PureLink™ ... PCR Purification Kit: Shipping Condition: Room Temperature: Test Time: 10 min: Videos. Can be performed in either single-column or 96-well plate format. High-quality RNA in less than 20 minutesUp to 1,000 ug of purified RNA from a single extraction. A full suite of products for all your DNA extraction needs. The PureLink™ Quick Gel Extraction and PCR Purification Combo Kit is designed to purify DNA fragments from agarose gels. PureLink® PCR Purification Kits provide rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR products... Shop Invitrogen™ PureLink™ ... 50 PureLink™ PCR Spin Columns with Collection Tubes; 50 PureLink™ Elution Tubes (1.7mL) 50 Preps: 1004.00 NOK K3100-01 and K3100-02 ... the kit). Provides two binding buffer options for fragments >100 bp or >300 bp. Specifications. Add 50 µL Elution Buffer to the center of the column. High-quality RNA in less than 20 minutesUp to 1,000 ug of purified RNA from a single extraction. PureLink™ Quick Gel Extraction & PCR Purification Combo Kit offers the ability to perform both a gel extraction or a PCR purification a single kit. The PureLink™ Quick Gel Extraction and PCR Purification Combo Kit is designed to purify DNA fragments from agarose gels. PureLink® PCR Purification Kits provide rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR products ... PCR Purification Kit: Shipping Condition: Room Temperature: Test Time: 10 min: Isolation Technology: Silica Spin Column: SDS. Catalog numbers . DNA & RNA Purification & Analysis, DNA Extraction, PCR Product Clean-Up. Store at room temperature. Description. Minor changes were made to the protocol supplied with the Invitrogen PureLink® PCR Purification Kit. System Overview The PureLink® Plant Total DNA Purification Kit is based on the selective binding of dsDNA to silica-based membrane in the presence of chaotropic salts. To begin with, the PureLink™ Kit comes with 2 Binding Buffers. The PureLink Viral RNA/DNA Mini Kit accommodates starting volumes of up to 500 µL, and elution volumes as low as 10 µL; The PureLink Pro 96 Viral RNA/DNA Kit can be used on a vacuum manifold or centrifuge, requires a minimum speed of just 2,250xg, and uses a bucket depth of only 50 mm, eliminating the need for high speed … Can be performed in either single-column or 96-well plate format. DNA & RNA Purification & Analysis, DNA Extraction, PCR Product Clean-Up. Our DNA extraction products include kits and reagents for the purification of cell-free DNA, microbial DNA, PCR clean-up, and sequence-specific DNA. Provides two binding buffer options for fragments >100 bp or >300 bp. Ensure that the DNA fragment of interest is completely SDS. The isolated DNA is 20–50 kb in size and is suitable for PCR, restriction enzyme digestion, and Southern blotting. Documents. Get All The Latest News Help & Support Center › English Change country; 0. PureLink PCR Purification kit (Invitrogen, Carlsbad, CA, USA) was used to extract DNA from the cysts according to the recommendations of the manufacturer [23]. The plant lysate is prepared using Resuspension Buffer (R2) Sensitive. This product (s) resides … PureLink ® Spin Column. The PureLink PCR Purification Kit is based on the selective binding of dsDNA to silica-based membrane in the presence of chaotropic salts. Simply mix the PCR product with Binding Buffer and apply to the PureLink Spin Column. The kit uses proven PureLink spin column technology for robust yields of purified DNA ready for downstream PCR, sequencing, or other applications. The PureLink® RNA Mini Kit provides rapid column based purification of total RNA from a wide range of cell and tissue types. View tips and tricks on selecting the right purification system and how to set up your plasmid purification system in order to obtain maximum yield for downstream experiments. The Ambion PureLink miRNA Isolation Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total microRNA (miRNA) from a wide variety of sources without the need for hazardous reagents such as phenol. Documents. The PureLink™ Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. PureLink® PCR Purification Kits provide rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR products... Shop Invitrogen™ PureLink™ English Change country. Our DNA extraction products include kits and reagents for the purification of cell-free DNA, microbial DNA, PCR clean-up, and sequence-specific DNA. The kit is available in sizes of 50 reactions, 250 reactions and also in a 96-well plate format (enough for 4 x 96 well plates). The Ambion PureLink miRNA Isolation Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total microRNA (miRNA) from a wide variety of sources without the need for hazardous reagents such as phenol. Isolate and purify high-quality genomic DNA from a variety of DNA types. Materials. Bioz Stars score: 99/100, based on 1 PubMed citations. Get All The Latest News Help & Support Center › English Change country; 0. The PureLink™ Pro 96 PCR Purification Kits provide rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR products. Takes <10 min. Reagents. PCR Purification Kit is based on the selective binding of dsDNA to silica-based membrane in the presence of chaotropic salts. When you use the kit, you will mix a PCR product with Binding Buffer to adjust conditions for subsequent dsDNA binding to the PureLink. Determine the quality and quantity of your RNA by UV absorbance at 260 nm. The PureLink Genomic Plant DNA Purification Kit allows efficient isolation of total DNA in about 40 minutes from a variety of plant samples, such as spinach, tomato, soy, wheat, Arabidopsis, and mushroom. For long–term storage, keep the purified RNA at –80°C. The simple PureLink™ bind/wash/elute procedure facilitates routine purifications of double-stranded DNA fragments from 100bp to 12kb. Various concentrations of Armored RNA HCV were prepared in human plasma and 200 uL of the plasma was used for viral RNA purification with both the PureLink Pro 96 viral DNA/RNA purification system and the PureLink Viral RNA/DNA Mini Kit. A fragment of 753 bp (base pairs) from the bg gene was amplified with the primers G7 (5′AAGCCCGACGACCTCACCCGCAGTGC3′) and G759 … Choose the Kit That Best Suits Your Needs. To get DNA of high quality from PCR products, we use this kit. The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits. PureLink® PCR Purification Kit . Info:Supplier Page View Product Specs View Product. The figure below shows a DNA Ladder (Lane 1), the PCR product before purification (Lane 2), and the PCR product after purification (Lane 3). The kit is designed to efficiently isolate genomic DNA from mammalian cells and tissues,mouse tail, E. coli cells, and yeast. The kit uses proven PureLink spin column technology for robust yields of purified DNA ready for downstream PCR, sequencing, or other applications. PureLink Genomic Plant DNA Purification Kit: PureLink Pro 96 Genomic DNA Purification Kit: MagMAX DNA Plant Isolation: Plant tissue input: 100 mg: 100 mg: up to 25 mg: up to 100 mg ... remove inhibitors inherent in plant samples that can carry over into the final product and interfere with downstream PCR applications. The PureLink HQ Mini Plasmid Purification Kit is designed for the isolation of high-quality plasmid DNA that is suitable for restriction enzyme digestion, PCR, sequencing, bacterial cell transformation, and mammalian cell transfection. The PureLink™ Genomic DNA Purification Kit allows rapid and efficient purification of genomic DNA. The QIAquick PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products >100 bp. Larger DNA fragments may be purified from gels using these kits, but may result in lower DNA recovery. PureLink Viral RNA/DNA Mini Kit. Takes <10 min. Check the amplicon on a gel to verify the PCR product prior to purification. Our lab performs a lot of cloning to create or optimize different lentiviral constructs. Catalog Number: K3100-01 . Thermo Fisher purelink pcr purification kit Purelink Pcr Purification Kit, supplied by Thermo Fisher, used in various techniques. The PureLink™ Microbiome DNA Purification Kit enables fast purification of high-quality microbial and host DNA from a wide variety of sample types, including challenging samples such as stool and soil. Storage & Stability. The PureLink® RNA Mini Kit provides rapid column based purification of total RNA from a wide range of cell and tissue types. Pure. ZERO BIAS - scores, article reviews, protocol conditions and more Benefits: Yields highly concentrated viral nucleic acids for greater sensitivity in detection - start with 500 µl of sample and elute in just 10 µl volume ®The PureLink Quick Gel Extraction and PCR Purification Combo Kit is suitable for purifying DNA fragments from 40 bp–10 kb in size. Materials. PureLink® PCR Purification Kits provide rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR products... Shop Invitrogen™ PureLink™ English Change country. Wash Buffer (supplied with kit) Elution Buffer (10 mM Tris-HCl, pH 8.5) Disstilled water >7.0 pH. Collection Tube. PCR Purification Kit as described in this manual. Using the kit, plasmid DNA can be isolated from varying amounts of bacterial cells. Certificates Special Offers. The PureLink™ Microbiome DNA Purification Kit enables fast purification of high-quality microbial and host DNA from a wide variety of sample types, including challenging samples such as stool and soil. * It should be noted that our products are only used for research, not for clinical use. The PCR product was analyzed by agarose gel electrophoresis and shows efficient purification of a 965-bp PCR product from a 232-bp PCR fragment and primers. System Overview The PureLink™ 96 PCR Purification Kit is based on the selective binding of dsDNA to silica-based membrane in the presence of chaotropic salts. The simple procedure uses a silica-based spin cartridge to purify DNA fragments from 40 bp to 10 kb in < 30 minutes.
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